Bernhard Sonnleitner's Advances in Biochemical Engineering - Biotechnology. - PDF

By Bernhard Sonnleitner

With contributions through various specialists

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Amperometric transducers measure the current (flux of electrons) caused by oxidation or reduction of the species of interest when a voltage is applied between the working and the reference electrode. g. g. [169]. Other determinations focus on the Instrumentation of Biotechnological Processes 33 detection of H2 O2 or NADH [9]. Sensors are currently being made smaller and smaller [297, 298]. Potentiometric transducers measure the potential between the sensing element and a reference element. Thus, in contrast to amperometric transducers, practically no mass transport occurs; the response depends on the development of the thermodynamic equilibrium.

This method is much faster than all of the counting techniques (and is again a solution of an inverse problem). 4 Cellular Components or Activities The trick for analyzing a biocatalytic activity must also be exploited whenever the medium contains particulate matter because this, of course, would falsify any direct gravimetric determination. Herbert [165] also gives an overview of many different indirect methods. One such often-used method is the measurement of dehydrogenase activity, for example, INT dehydrogenase [117, 253, 254].

Sensors are currently being made smaller and smaller [297, 298]. Potentiometric transducers measure the potential between the sensing element and a reference element. Thus, in contrast to amperometric transducers, practically no mass transport occurs; the response depends on the development of the thermodynamic equilibrium. pH changes often correlate with the measured substance because many enzymatic reactions consume or produce protons. Conductometric transducers consist of two pairs of identical electrodes, one of which contains an immobilized enzyme.

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Advances in Biochemical Engineering - Biotechnology. - Bioanalysis and Biosensors for Bioprocess Monitoring by Bernhard Sonnleitner


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