By Phyllis R. Brown, Eli Grushka
An updated reference, supplying an leading edge standpoint on breakthroughs in separation technological know-how, akin to reverse-phase HPLC, advances in hyphenation, and linear dependence of relative retention values at the nature of the service gasoline and ordinary column strain in gas-liquid chromatography.
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Additional resources for Advances in Chromatography: Volume 41
In Fig. 2 the differences between these two detection modes are illustrated taking a packed capillary as an example. B. Propulsion of Mobile Phase Ideally, in CEC the mobile phase is driven through the chromatographic bed exclusively by the effect of electroosmosis. Electroosmosis is an electrokinetic effect originating from the electrophoretic movement of the diffuse layer of the electric double layer that is formed at the interface between the liquid and the solid. In CE, electroosmosis is generated only at the electric double layer formed Fig.
Steiner et al. report a loss in efficiency of 50–60% when increasing the inner diameter from 100 to 180 µm. Banholczer and Pyell  report that the retention factors for several noncharged solutes determined for a packed capillary (inner diameter ϭ 100 µm) by CEC with thiourea as marker of the holdup time were independent of the concentration of buffering salts (NaH 2 PO 4 ϩ Na2 HPO 4 ) in the mobile phase (see Fig. 12). 01 to 7 mmol/L. If there was a significant temperature rise inside the capillary due to Joule heating, a decrease in retention factors with increasing concentration of the buffering salts would be expected.
F. Bubble Formation In the early days of CEC, bubble formation during a chromatographic run was the most important practical problem that hampered the widespread use of this technique. ’’ They recommended thermostating of columns at temperatures close to ambient or op- 28 / Pyell erating the whole column under pressure as the most effective preventive measures. There are indications, however, that self-heating is not the primary cause of the formation of bubbles in the packing during a chromatographic run.
Advances in Chromatography: Volume 41 by Phyllis R. Brown, Eli Grushka