By Paul L. Dubin
The swift improvement of recent packings for aqueous size-exclusion chromatography has revolutionized this box. excessive solution non-adsorptive columns now make attainable the effective separation of proteins and the fast and particular selection of the molecular weight distribution of man-made polymers. This expertise can be being utilized to the separation of small ions, the characterization of associating platforms, and the dimension of branching. while, basic experiences are elucidating the mechanisms of many of the chromatographicprocesses. those advancements in rules and functions are assembled for the 1st time during this e-book. basic matters are handled: the jobs of pore constitution and macromolecular dimensions, hydrophobic and electrostatic results, and the selection and keep an eye on of column potency. High-performance packings according to derivatized silica are reviewed intimately. unique recommendations are completely defined, together with SEC/LALLS, inverse exclusion chromatography, and frontal sector chromatography.
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The anomer e f f e c t was not as s t r i k i n g as expected although somewhat g r e a t e r s o l u b i l i t y - i n c r e a s i n g e f f e c t o f 8-methylglycosides than a-methylglycosides appears t o be s i g n i f i c a n t . The p o s i t i v e cosolvent e f f e c t o f maltodextrins on the aqueous s o l u b i l i t i e s o f alkanols and aromatic hydrocarbons increases w i t h increase i n t h e number o f glucose residues ( r e f . 24,28). , t h e dominant e f f e c t o f AFint over AFCaV.
Tl] O t h e r values a r e compiled by Yau e t a l . constants ( r e f . 7). 443#0'42 Corresponding d a t a f o r p o l y e t h y l e n e g l y c o l a t 25OC and f o r t h e m o l e c u l a r w e i g h t range 200-8000, t a k e n f r o m a r e v i e w by B a i l e y and Koleske ( r e f . 156M0'50 (9) The d a t a a r e t h e n p l o t t e d as l o g [ V I M versus Ve, as shown i n F i g u r e 2. When t h e t e s t sample i s examined i n t h e same column, t h e p r o d u c t [ V I M can be read d i r e c t l y f r o m t h e c a l i b r a t i o n curve.
16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. J. , 16 (1967) 838-840. D. Bly, i n B. ), Gel Permeation Chromatography i n Polymer Chemistry, P h y s i c a l Methods i n Macromolecular Chemistry, Vol 2, Dekker, New York, 1972. W. J. D. Bly, Modern S i z e E x c l u s i o n Chromatography, W i l e y , New York, 1979. E. K. Oh, u n p u b l i s h e d o b s e r v a t i o n s (1984). M. Duval, B. B l o c h and S.
Aqueous Size-Exclusion Chromatography by Paul L. Dubin