By Sidney Fleischer, Becca Fleischer (Eds.)
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Extra info for Biomembranes Part M
Bochkov, Tetrahedron 23, 693 (1967).  ALKYLGLYCOSIDE DETERGENTS: SYNTHESIS AND USE 31 detergent peaks are located by adding 1 ml 5% aqueous phenol to 100-/~1 aliquots of each tube (cloudiness indicates dodecanol is present) followed by 5 ml concentrated sulfuric acid (orange-brown color indicates detergent). The alcohol runs ahead of the detergent and is usually found in the initial 1200 ml. 38). The a- and/3-1aurylmaltoside fractions are pooled separately and any overlapping region can be concentrated and added to the next sample to be chromatographed.
1982. 4 D. A. Thompson and S. Ferguson-Miller, Biochemistry 22, 3178 (1983). 5 p. Knudsen and W. L. Hubbell, Membr. Biochem. 1, 297 (1978). 6 M. W. Kendall-Tobias and M. Seibert, Arch. Biochem. Biophys. 216, 255 (1982). -, ~< o< _,,-. ~ t-. 2: ~J •= . I ~ 8 < 8~8~28 = = 88 ~ = b ~~ 30 GENERAL APPROACHES  iodine, and 200 g Drierite, in that order. The mixture is stirred for 12 hr with a propeller-type stirrer inserted through the neck of the vessel. 38). The reaction mixture is filtered through a pad of celite on a 2-liter scintered glass funnel (small pore) and washed with 1000 ml dichloromethane.
Biochem. 73, 125 (1977). 35 H. Rottenberg and A. Scarpa, Biochemistry 13, 4811 (1974). 16 GENERALAPPROACHES   I s o l a t i o n o f H i g h l y C o u p l e d H e a r t M i t o c h o n d r i a in H i g h Yield Using a Bacterial Collagenase By PETER P. SHELAGH M. FERGUSON-MILLER, and CLARENCE H. SUELTER TOTH, Introduction Numerous methods have been developed for the isolation of mitochondria from a variety of tissues. 1,2 These procedures usually involve homogenization of the tissue by mechanical means, and separation of the mitochondria containing fraction by differential centrifugation.
Biomembranes Part M by Sidney Fleischer, Becca Fleischer (Eds.)