Biomembranes Part M - download pdf or read online

By Sidney Fleischer, Becca Fleischer (Eds.)

ISBN-10: 0121820254

ISBN-13: 9780121820251

The severely acclaimed laboratory normal, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - really an important booklet for researchers in all fields of existence sciences

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Bochkov, Tetrahedron 23, 693 (1967). [3] ALKYLGLYCOSIDE DETERGENTS: SYNTHESIS AND USE 31 detergent peaks are located by adding 1 ml 5% aqueous phenol to 100-/~1 aliquots of each tube (cloudiness indicates dodecanol is present) followed by 5 ml concentrated sulfuric acid (orange-brown color indicates detergent). The alcohol runs ahead of the detergent and is usually found in the initial 1200 ml. 38). The a- and/3-1aurylmaltoside fractions are pooled separately and any overlapping region can be concentrated and added to the next sample to be chromatographed.

1982. 4 D. A. Thompson and S. Ferguson-Miller, Biochemistry 22, 3178 (1983). 5 p. Knudsen and W. L. Hubbell, Membr. Biochem. 1, 297 (1978). 6 M. W. Kendall-Tobias and M. Seibert, Arch. Biochem. Biophys. 216, 255 (1982). -, ~< o< _,,-. ~ t-. 2: ~J •= . I ~ 8 < 8~8~28 = = 88 ~ = b ~~ 30 GENERAL APPROACHES [3] iodine, and 200 g Drierite, in that order. The mixture is stirred for 12 hr with a propeller-type stirrer inserted through the neck of the vessel. 38). The reaction mixture is filtered through a pad of celite on a 2-liter scintered glass funnel (small pore) and washed with 1000 ml dichloromethane.

Biochem. 73, 125 (1977). 35 H. Rottenberg and A. Scarpa, Biochemistry 13, 4811 (1974). 16 GENERALAPPROACHES [2] [2] I s o l a t i o n o f H i g h l y C o u p l e d H e a r t M i t o c h o n d r i a in H i g h Yield Using a Bacterial Collagenase By PETER P. SHELAGH M. FERGUSON-MILLER, and CLARENCE H. SUELTER TOTH, Introduction Numerous methods have been developed for the isolation of mitochondria from a variety of tissues. 1,2 These procedures usually involve homogenization of the tissue by mechanical means, and separation of the mitochondria containing fraction by differential centrifugation.

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Biomembranes Part M by Sidney Fleischer, Becca Fleischer (Eds.)

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