New PDF release: Biothiols

By Packer L.

This specific, cutting-edge reference offers an in depth account of the molecular mechanisms underlying the a number of services of biothiols-emphasizing the organic and medical implications of interactions among oxidants and biothiols.

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Asmus, L. G. Forni, and R. L. Willson, Int. J. Radiat. Biol. 52, 589 (1987). 22 R. L. Willson, J. Chem. , Chem. , 1425 (1970). [4] KINETICS OF THIOL REACTIONS 49 anion k12 = 9 × 10s M -1 sec -a (Ref. 22)]. Thus the contamination of solutions with molecular oxygen could eventually lead to an underestimation of the actual yields of thiyl radicals detected directly or indirectly. RS. + O2 ~ RSOO. ),23 monomeric (>S +. 2s Stopped-Flow Measurements Nitric oxide, NO-, is an important cellular messenger that often acts via nitrosylation of protein thiol groups.

SR]- (10) These complexes strongly absorb near/in the visible region with Amaxbeing located between 390 and 500 nm, exhibiting extinction coefficients between 2990 and 9200 M-1 cm-1,10,19 depending on the actual structure of the thiol. The presence of sufficient amounts of deprotonated thiol can thus be utilized for a more sensitive, although indirect, detection of generated thiyl radicals. It should be noted, however, that many carbon-centered radicals do not react or react only slowly with thiolate anions.

Biol. Chem. 260, 1601 (1985). 38 L. G. Forni, V. O. Mora-Arellano, J. E. Packer, and R. L. Willson, J. Chem. , Perkin Trans. 2, 1579 (1988). 39 B. C. Gilbert, in "Sulfur-Centered Reactive Intermediates in Chemistry and Biology" (C. -D. ), p. 135. Plenum, New York, 1990. 4o M. D. Sevilla, D. Becker, and M. Yan, Int. J. Radiat. Biol. 57, 65 (1990). ) have been subject to extensive investigation whereas ESR spectra of the thiyl radical itself are generally hardly observable or difficult to analyze.

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Biothiols by Packer L.

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