By John N. Abelson, Melvin I. Simon, Helmut Sies
The significantly acclaimed laboratory typical for 40 years, Methods in Enzymology is among the such a lot hugely revered guides within the box of biochemistry. when you consider that 1955, each one quantity has been eagerlyawaited, often consulted, and praised via researchers and reviewers alike. greater than 250 volumes were released (all of them nonetheless in print) and masses of the fabric is correct even today--truly a necessary e-book for researchers in all fields of lifestyles sciences. Key good points * Prevention of oxidative rigidity * Redox law of metabolism * Toxicology * Carcinogenesis
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Extra info for Biothiols, Part A: Monothiols and Dithiols, Protein Thiols, and Thiyl Radicals
For a dithiol system in which the dithiol incorporates two labels per mole of protein and the mixed disulfide incorporates one label per mole of protein, the radiolabel incorporated per mole of proteins will then be given by [Label] _ 2R[GSH] + Kmix[GSH] [Ptot] R[GSH] + Kmix[GSH] + Kox (18) where the terms in the numerator represent the incorporation of label into dithiol and the mixed disulfide intermediate. Complex Equilibria The description of the equilibrium behavior of test thiols and dithiols provided above is based on simplifications and assumptions regarding the types of disulfides formed at equilibrium.
P. Szajewski and G. M. Whitesides, J. A m . Chem. Soc. 102, 2011 (1980). 10 OVERVIEW ,  If the test system has two sulfhydryl groups that are in close enough proximity, the initially formed mixed disulfide may be displaced by an intramolecular reaction leading to the formation of an intramolecular disulfide. While the overall reaction is complicated by the potential accumulation of multiple redox isomers (see Complex Equilibria), in practice, the intramolecular reaction is often so favorable that negligible mixed disulfide species are present at equilibrium.
Res. Commun. 161, 113 (1989). 5 C, Sch6neich,U. Dillinger,F. -D. Asmus,Arch. Biochem. Biophys. 292, 456 (1992). 6 p. O'Neill, Radiat. Res. 96, 198 (1983). METHODS IN ENZYMOLOGY,VOL. 251 Copyright© 1995by AcademicPress, Inc. All rightsof reproductionin any form reserved. 32 THIYL RADICALS [31 Xenobiotic radicals produced by one-electron oxidation of drugs are often reactive toward thiols. Examples include aromatic amines, phenothiazines, or phenols. + + R S H ~ , ~ P Z + RS. + H + (3) Equation (3) contrasts with Eq.
Biothiols, Part A: Monothiols and Dithiols, Protein Thiols, and Thiyl Radicals by John N. Abelson, Melvin I. Simon, Helmut Sies