New PDF release: Cardiac Tissue Engineering: Methods and Protocols

By Milica Radisic, Lauren D. Black III

ISBN-10: 1493910469

ISBN-13: 9781493910465

ISBN-10: 1493910477

ISBN-13: 9781493910472

Cardiac Tissue Engineering: tools and Protocols presents a set of protocols on cardiac tissue engineering from pioneering and major researchers around the world. those comprise equipment and protocols for cellphone education, biomaterial guidance, phone seeding, and cultivation in quite a few platforms. Written within the hugely winning Methods in Molecular Biology sequence layout, chapters contain introductions to their respective issues, lists of the required fabrics and reagents, step by step, comfortably reproducible laboratory protocols, and key tips about troubleshooting and averting identified pitfalls.

Authoritative and sensible, Cardiac Tissue Engineering: equipment and Protocols highlights the key concepts, either experimental and computational, for the research of cardiovascular tissue engineering.

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Additional resources for Cardiac Tissue Engineering: Methods and Protocols

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Wells marked “n/a” do not contain cells, nor media. The grey boxes represent the theoretical line at which wells containing only a single cell are reached 5. For clonal expansion, dilute cells to 10,000 cells/mL in culture media (see Note 8). 6. Prepare a 96-well plate by adding 100 μL of culture media to each well, except for A1 (see Note 9). 7. , 2,000 cells) and place it in well A1 of a 96-well plate. 8. Transfer 100 μL from A1 to B1, and mix by pipetting. 9. Transfer 100 μL from B1 to C1, and mix by pipetting.

J Mol Cell Cardiol 51:187–197 21. Mackie AR, Losordo DW (2011) CD34positive stem cells: in the treatment of heart and vascular disease in human beings. Tex Heart Inst J 38:474–485 22. Yang J, Ii M, Kamei N et al (2011) CD34+ cells represent highly functional endothelial progenitor cells in murine bone marrow. PLoS One 6:e20219 23. Garmy-Susini B, Varner JA (2005) Circulating endothelial progenitor cells. Br J Cancer 93: 855–858 24. Peichev M, Naiyer AJ, Pereira D et al (2000) Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors.

Favre J, Terborg N, Horrevoets AJ (2013) The diverse identity of angiogenic monocytes. Eur J Clin Invest 43:100–107 19. Kalka C, Masuda H, Takahashi T et al (2000) Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci U S A 97: 3422–3427 20. Kuraitis D, Hou C, Zhang Y et al (2011) Ex vivo generation of a highly potent population of circulating angiogenic cells using a collagen matrix. J Mol Cell Cardiol 51:187–197 21. Mackie AR, Losordo DW (2011) CD34positive stem cells: in the treatment of heart and vascular disease in human beings.

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Cardiac Tissue Engineering: Methods and Protocols by Milica Radisic, Lauren D. Black III


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