By Wojciech Gorczyca
Cytogenetics, fluorescence in situ hybridization (FISH) and molecular exams, specially polymerase chain response (PCR), play a tremendous function within the administration of sufferers with hematologic malignancies by way of supporting to set up the analysis, in addition to are expecting diagnosis, reaction to remedy and affliction development. Chromosomal and molecular abnormalities give you the best standards for type of hematopoietic tumors and sometimes contain the root for distinct therapy.
Cytogenetics, FISH and Molecular trying out in Hematologic Malignancies, offers a assessment of chromosomal and molecular alterations in hematologic malignancies and correlates the karyotypic and genetic abnormalities with morphology, immunophenotype and medical facts. With over a hundred and eighty figures and diagnostic algorithms, this article is key examining for all pathologists, hematopathologists, hematologic oncologists, cytogenetists, cytogenetic technologists and mobile biologists.
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Extra info for Cytogenetics, FISH and Molecular Testing in Hematologic Malignancies
Abnormalities of the short arm of chromosome 12 (12p) are found in about 5% of AMLs and are often reported in secondary leukemias, especially after prior mutagenic exposure. 252 Del(12p) present as a sole abnormality 39 ● ● ● B-CLL MCL B-cell lymphomas, high grade Plasma cell myeloma/MGUS T-cell lymphomas CMPDs (P. vera, ET, CIMF) Deletions of the long arm of chromosome 13 occur in myeloid and lymphoid tumors, with myeloid breakpoints more often involving the RB1 gene locus, while more lymphoid breakpoints fall telomeric of RB1.
Loss of the short arm of chromosome 17 is associated with a p53 mutation on the remaining allele in several hematopoietic malignancies. 11) is associated with a poorer prognosis in many hematopoietic malignancies. 9 Multiple myeloma with complex karyotype including monosomy 13 and monosomy 17. 247 The p53 (TP53) mutations are found in the majority of MDS patients with 17p deletion. 1); cytogenetics (partial Gorczyca-Ch02 42 11/12/07 3:15 PM Page 42 CYTOGENTICS, FISH AND MOLECULAR TESTING IN HEMATOLOGIC MALIGNANCIES lymphomas.
34 illustrates the gating strategy applied in the FC analysis of peripheral blood and bone marrow. Based on the intensity of CD45 staining (x-axis) and side scatter (SSC, y-axis) one can distinguish several major populations in normal Red cells are lysed and do not appear on flow cytometry dot plots Granulocytes (grey dots) have dimmer expression of CD45 (X-axis), than lymphocytes and monocytes, but display much higher side scatter. The latter corresponds to cytoplasmic granularity Blasts have dim to moderate expression of CD45 (X-axis) and low to minimally increased side scatter (Y-axis) Side scatter Plasma cells are CD45 negative and have low side scatter Monocytes (blue dots) have bright CD45 expression (X-axis) and mildly increased side scatter (Y-axis), when compared to lymphocytes.
Cytogenetics, FISH and Molecular Testing in Hematologic Malignancies by Wojciech Gorczyca