By L. A. Rozenszajn, J. Goldman, H. Poran, M. M. Werber, D. Shoham, J. Radnay (auth.), S. J. Baum, Dov H. Pluznik, L. A. Rozenszajn (eds.)
Read Online or Download Experimental Hematology Today—1985: Selected Papers from the 14th Annual Meeting of the International Society for Experimental Hematology, July 14–18, 1985, Jerusalem, Israel PDF
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Extra info for Experimental Hematology Today—1985: Selected Papers from the 14th Annual Meeting of the International Society for Experimental Hematology, July 14–18, 1985, Jerusalem, Israel
Of 2 to 6 experiments. IL-3 was added at the start of the incubation period at a concentration of 20 u/ml. Control cultures contained only RMPI medium and 10% FCS. M. 2 antibody and complement (C') before assay for day 11 CFU-S or before culture in vitro with IL-3 (20 u/ml) for 96 hours. M. 005 Marrow cells were photosensitized by exposure to MC 540 (15~g/ml) and light for 45 or 60 minutes, washed and cultured with or without IL-3 (20 u/ml). After 96 hours the cells harvested for assay of day 11 CFU-S.
As demonstrated previously (1). 2 antibody and complement did not influence the recovery of day 11 CFU-S (Table 4). Exposure of marrow ce 11 s to the Thy 1. 2 ant i body and comp 1ement before incubation of the cells with the lymphokine for 96 hours also did not diminish the number of CFU-S recovered as compared to cells not treated with the antibody. Thus. cells bearing the Thy 1. 2 ant i gen were not requ i red for the interaction of IL-3 and day 11 CFU-S. Spleen colony forming units are heterogeneous with respect to their capacity for marrow repopulation and self-renewal (12).
I. Stimulation of haemopoietic stem cell proliferation characteristics of the stimulator producing cells. Leuk. Res. ~, 531, 1982. Interaction of Interleukin 3 with Pluripotent Hematopoietic Stem Cells Jerry L. Spivak l , Robert R. L. Smith2 , and James N. Ihle 2 IDivision of Hematology, Department of Medicine and the Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA une National Cancer Institute, Frederick Cancer Research Facility, Frederick, Maryland, USA I NTRODU cn ON IL-3 appears to act directly on CFU-S rather than through accessory cells.
Experimental Hematology Today—1985: Selected Papers from the 14th Annual Meeting of the International Society for Experimental Hematology, July 14–18, 1985, Jerusalem, Israel by L. A. Rozenszajn, J. Goldman, H. Poran, M. M. Werber, D. Shoham, J. Radnay (auth.), S. J. Baum, Dov H. Pluznik, L. A. Rozenszajn (eds.)