New PDF release: Handbook of methods and instrumentation in separation

By C F Poole; Ian D Wilson

ISBN-10: 0123750954

ISBN-13: 9780123750952

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Extra resources for Handbook of methods and instrumentation in separation science Volume 1

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Consequently longer operational cycles and higher flows result. One limitation of the expanded bed system is that adsorption can only be carried out in one stage, resulting in a less efficient process. 32 Affinity Separations Expanded beds are only an intermediate stage towards fluidized beds. Several variations of fluidized bed technology have been adopted to evaluate them for affinity processing. One example is the use of perfluorocarbon emulsions in a countercurrent contactor. The affinity perfluorocarbon emulsion is loaded with crude source material into the base of a column in a similar manner to that of an expanded bed.

The optimal range of Kd for affinity chromatography lies between 10À4 and 10À8 mol LÀ1. Most biological ligands can be used for affinity purposes providing they can be immobilized, and once immobilized continue to interact successfully with their respective ligates. The ligand can be naturally occurring, an engineered macromolecule or a synthetic molecule. Table 1 provides some examples of immobilized ligands used to purify classified proteins. The affinity method is not restricted to protein separations; nucleic acids and whole cells can also be separated.

In contrast, combinatorial library design is now thought by some to provide the best opportunity of discovering new novel peptides and small molecule structures for pharmaceutical application. A quite natural extension of the concept is to use combinatorial libraries to discover ligands capable of achieving highly efficient protein separations. When directed at drug discovery the earliest workers built libraries from peptides. For ligands libraries will generally utilize simple chemical molecules and occasionally smaller peptides.

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Handbook of methods and instrumentation in separation science Volume 1 by C F Poole; Ian D Wilson


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