By Reiner Salzer, Heinz W. Siesler
This moment version of the winning prepared reference is up to date and revised with nearly 30% new content material to mirror the varied instrumental advancements and enhancements, in addition to the numerous growth of this swiftly constructing box. for instance, the mix of IR imaging with AFM has improved the a possibility lateral answer through an order of significance right down to a number of hundred nanometers, thus launching a multiplicity of recent functions in fabric technology. additionally, Raman and IR spectroscopic imaging became key applied sciences for the existence sciences and this day give a contribution enormously to a greater and extra precise figuring out of diverse organic and clinical examine issues. The topical constitution of this re-creation is now subdivided into 4 components. the 1st treats the basics of the instrumentation for infrared and Raman imaging and mapping and an outline at the chemometric instruments for photo research. the second one half describes a large varie-ty of functions starting from biomedical through nutrition, agriculture and vegetation to polymers and prescribed drugs. this can be by means of an outline of imaging strategies working past the diffraction restrict, whereas the ultimate half covers exact methodical advancements and their application in particular fields. With its many precious functional counsel, it is a must-have review for researchers in educational and business laboratories wishing to procure trustworthy effects with this method. Read more...
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Additional resources for Infrared and Raman spectroscopic imaging
2 Mid-Infrared Microspectroscopy and Mapping Cassegrain condenser. The condenser focuses the beam into a small spot where the sample is mounted. The radiation that is transmitted through the sample is collected by a Cassegrain objective and refocused at a remote adjustable aperture. The part of beam that passes through the aperture is imaged onto an optical viewer or, more frequently today, a video camera, so that the visible image of the sample can be viewed. The sample is usually mounted on an x, y, z stage.
Reproduced from Ref. . ) (see below). 4. 5). 5 Percent of stray energy outside the area deﬁned by an 8 μm × 240 μm rectangular aperture for various imaging modes. (Reproduced with permission from Ref. . 6 Dual confocal aperturing achieved with a single physical aperture. (Reproduced from Ref. . ) It was found that keeping the apertures of a dual-aperture microscope in alignment over a long period of use was not an easy task, especially when high spatial resolution is desired and the apertures must be very small.
The IRE is spring loaded to avoid damage, and there are ﬁve diﬀerent pressure steps to allow optimal contact for hard and soft samples. 4). Silicon is rarely used for the fabrication of large IREs, for which the optical path may be several centimeters, because the presence of trace impurities leads to excessive absorption of the radiation below about 1200 cm−1 . With the very short path through the IRE installed in a microscope objective, however, this is no longer a problem. Thus, either silicon or germanium can be used productively as the IRE in an ATR objective.
Infrared and Raman spectroscopic imaging by Reiner Salzer, Heinz W. Siesler