New PDF release: Methods in Cell Biology, 17

By D Marshall Prescott

ISBN-10: 0080859143

ISBN-13: 9780080859149

ISBN-10: 0125641176

ISBN-13: 9780125641173

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Add drop by drop equal volume of solution F (gentle Vortex). Layer lysate onto 5 volumes G (wide-bore polystyrene pipetteb). I Remove upper part of sup from surface by pipette; wipe inside tube and remove remainder of sup by pipette and discard 3000 g for 15 minutes (swing-out rotor) Take up pellet intact in same volume of solution E used above; relayer on solution G as above in Dounce homogenizer' (pipette as above). 2 mM dithiothreitol by 5-20 strokes of tight piston. 5 hours Yield (as DNA) 'All operations are at 4OC.

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Biol. 34, 305 (1968). 12. , Gilmour, R. , More, I. A. , Karolinska Symp. Res. Methods Reprod. Endocrinol. 5, 277 (1972). 13. , Potter, V. , and Morris, M. , Cancer Res. 30,776 (1970). 14. , Choi, Y. , Olson, M. 0. , Ro-Choi, T. , and Yeoman, L. , Karolinska Symp. Res. Methods Reprod. Endocrinol. 5, 35 (1972). 15. MacGillivray, A. , Biochim. Biophys. Acta 277, 384 (1972). 16. , Gilmour, R. , Birnie, G. , Harrison P. , Windass, J. , and Young, B. , Cold Spring Harbor Symp. Quant. Biol. 38, 885 (1974).

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Methods in Cell Biology, 17 by D Marshall Prescott


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