By D Marshall Prescott
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Extra info for Methods in Cell Biology, 17
Add drop by drop equal volume of solution F (gentle Vortex). Layer lysate onto 5 volumes G (wide-bore polystyrene pipetteb). I Remove upper part of sup from surface by pipette; wipe inside tube and remove remainder of sup by pipette and discard 3000 g for 15 minutes (swing-out rotor) Take up pellet intact in same volume of solution E used above; relayer on solution G as above in Dounce homogenizer' (pipette as above). 2 mM dithiothreitol by 5-20 strokes of tight piston. 5 hours Yield (as DNA) 'All operations are at 4OC.
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Methods in Cell Biology, 17 by D Marshall Prescott