By Andre P. De Leenheer, Willy Lambert
3rd version collects and examines the large proliferation of knowledge on chromatographic research of fats and water soluble supplementations over the past decade. generally describes pattern guidance and ultimate size.
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All Rights Reserved. for the separation and recovery of seven carotenoids using methanol- and acetonitrile-based solvents, either straight or modiﬁed with ethyl acetate or tetrahydrofuran. Methanol-based solvents provided better recoveries, and polymeric C18 phases were better than monomeric C18 phases for separation (180). The effects of mobile-phase modiﬁers, modiﬁer concentration, and column temperature on the separation of seven carotenoids (lutein, zeaxanthin, β-cryptoxanthin, echinenone, α-carotene, β-carotene, and lycopene) on a polymeric C18 column were investigated (42).
From Ref. ) 45 Copyright 2000 by Marcel Dekker, Inc. All Rights Reserved. 46 ϫ 25 cm) using a mobile phase of methanol (92%) and water/ acetonitrile (3 :1, 8%) has been described (207,208). 1% triethylamine has been described for studying the distribution of lutein and zeaxanthin in human retina (209). 3′-Epilutein and 3′-hydroxy-beta, epsilon-caroten-3′-one have been identiﬁed by HPLC on a silica-based nitrile bonded column with hexane/CH 2Cl 2 /CH3OH/N,N-diisopropylethylamine as the mobile phase, as have lutein and zeaxanthin in human and monkey retina (210).
TLC is in fact preferable to HPLC for analysis of radiochemical impurities (degradation products) in radiolabeled retinoids and carotenoids, since in HPLC some impurities might not be eluted and detected; radioactivity on thin-layer plates may be detected by autoradiography (exposure of the plate to x-ray ﬁlm (98)) or by cutting the TLC plate into short sections which can be counted individually in vials by liquid scintillation. 5 to 8 cm), commercially available plates are convenient for either adsorption (silica or alumina) or reversed-phase (C8 or C18) chromatography, using mobile phases as appropriate for adsorption or reversed-phase HPLC.
Modern chromatographic analysis of vitamins by Andre P. De Leenheer, Willy Lambert