By Roger Knowles, Eldor A. Paul, Jerry Melillo and Henry Blackburn (Eds.)
This publication is the 1st laboratory handbook to assemble simple systems for dimension of reliable and radioactive isotopes of nitrogen, with particular purposes to plant, soil, and aquatic biology. This bench-top reference supplies functional insurance of mass and emission spectrometry, nitrogen fixation, nitrification, and identity, natural nitrogen, and the radioactive isotope 13N. equipment are defined so that researchers can adapt them, with no assistance from open air references, to nearly any activity they might come across in investigations of nitrogen transformation processes.
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Extra info for Nitrogen Isotope Techniques
6b, and the nut is finger-tightened to compress the O-ring against the outside of the delivery tube. If the bulb is stored in a refrigerator, the reagent will retain its activity for at least 6 mo. One milliliter of this solution will oxidize about 2 mg NH4+-N to N2. 2. Liquid N2. 3. Description of Procedure With stopcock 2 and valve 2 (Fig. 6a) closed (valve 3 is also closed; stopcock 1 and valve 1 are open), insert a shell vial containing the NH4+-salt sample into the O-ring fitting on the conversion apparatus (Fig.
03-320-3E) is satisfactory. 7-mm stainless-steel rods are placed in each jar (Kelley and Cannon, 1990). Rotation of the bottles (1 00-130 rpm) may be accom plished with a roller mill (Harris and Paul, 1989), but the conveyor belt assembly described by Kelley and Cannon (1990) is more convenient for large numbers of samples, because up to 55 samples can be ground concur rently. Samples must be dried before grinding; moreover, plant materials should be ground to <1 mm (or finer) with a rotary mill.
1 g plant material, 1 g soil) is conveniently accomplished using polyethylene vials. D. Diffusion of NH4+-N 1. Principles Diffusion techniques are an alternative to steam distillation for separation and collection of NH4+-N (Hauck, 1982), and these techniques have special value in the preparation of samples for automated mass spectrometers, because only microgram quantities of N are required. Recent work by Brooks et al. (1989) has led to the development of a diffusion procedure for automated isotope ratio analysis of inorganic N in soil extracts.
Nitrogen Isotope Techniques by Roger Knowles, Eldor A. Paul, Jerry Melillo and Henry Blackburn (Eds.)