Download e-book for iPad: Tumor Aneuploidy by Thomas Büchner, Clara D. Bloomfield, Wolfgang Hiddemann,

By Thomas Büchner, Clara D. Bloomfield, Wolfgang Hiddemann, Dieter K. Hossfeld, Johannes Schumann, M. Andreeff, B. Barlogie, D.B. von Bassewitz, R. Becher, Clara Bloomfield, T. Büchner, F. Carbonell, B. Eagle, T.M. Fliedner, A. Ganser, W. Göhde, E. Grundmann

ISBN-10: 3540153764

ISBN-13: 9783540153764

ISBN-10: 3642704581

ISBN-13: 9783642704581

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55, D-4300 Essen 1, FRG 35 Tumor Aneufloidy Bürnner el a . © Springer-Verlag: Berlin Heidelberg 1985 ~ Shape, size (physicall f.! ---------. ONA '"""""'"""'""'" Karyotype (qualitative l Fig. 1. g. marker chromosomes (Abnormal chromosomes identified by an abnormal banding pattern and analysed according to their origin) Total Number of Chromosomes in Tumor Cells The majority of cytogenetic data published in the field of solid tumors refer to abnormal chromosome counts. The evaluation of the number of chromosomes per metaphase is a rather simple approach requiring no detailed banding analysis.

For staining cells were washed and suspended in a solution of combined ethidium bromide and mithramycin fluorescent dyes. Processing and staining procedures have been described in detail for solid tumors [11, 43] and blood or bone marrow [11, 16, 18]. As a ploidy standard nuc1eated blood cells of normal donors were admixed in two different concentrations and stained together with the 1 Medizinische Klinik und Poliklinik der Universität, Albert-Schweitzer-Str. 33, D-4400 Münster, FRG 41 Tumor Aneuploidy Büchner et aL © Springer-Verlag: Berlin Heidelberg 1985 sampie cells.

5 and 7, but also of No. 3 and 17 in 60% of the cases; marker chromosomes, particularly double minutes; multiclonality and, related to this, karyotypic changes during the course ofthe disease. In order to understand this astonishing phenomenon of such characteristic chromosomal changes in secondary acute leukemias, a number of questions remain to be elucidated. First of all the question comes up as to the karyotype of such leukemia-patients who had a primary malignancy in their history for which, however, they had received neither radio- nor chemotherapy.

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Tumor Aneuploidy by Thomas Büchner, Clara D. Bloomfield, Wolfgang Hiddemann, Dieter K. Hossfeld, Johannes Schumann, M. Andreeff, B. Barlogie, D.B. von Bassewitz, R. Becher, Clara Bloomfield, T. Büchner, F. Carbonell, B. Eagle, T.M. Fliedner, A. Ganser, W. Göhde, E. Grundmann


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